kbl [2018-11-23 18:46:59]

2-amino-3-ketobutyrate CoA ligase

locus: BSU17000

pI: 6.25

mw: 43.12 kDa

protein length: 392 aa

gene length: 1176 bp

function: threonine utilization

product: 2-amino-3-ketobutyrate CoA ligase

essential: no

ec: 2.3.1.29

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.8|Utilization of threonine/ glycine]

Gene

Coordinates: 1,771,517 → 1,772,695

The protein

Catalyzed reaction/ biological activity

• Acetyl-CoA + glycine = CoA + 2-amino-3-oxobutanoate (according to Swiss-Prot)

Protein family

• class-II pyridoxal-phosphate-dependent aminotransferase family (according to Swiss-Prot)

Modification

• pyridoxal-phosphate (according to Swiss-Prot)

Structure

• [PDB|1FC4] (from ''Escherichia coli'', 40% identity, 60% similarity) [Pubmed|11318637]

Expression and Regulation

Operons

• genes: [gene|8E64B62F15B92A2AACD68B913909100AE276D114|tdh]-[gene|1BC05699DEB3A16944FB8305F22976AED75805E8|kbl]
  description: [Pubmed|22383849]

Biological materials

Mutant

• BKE17000 (Δ[gene|1BC05699DEB3A16944FB8305F22976AED75805E8|kbl]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE17000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTATCCCCCTTTATG, downstream forward: _UP4_TAACAAGAGGAAGAGTTTAC
• BKK17000 (Δ[gene|1BC05699DEB3A16944FB8305F22976AED75805E8|kbl]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK17000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTATCCCCCTTTATG, downstream forward: _UP4_TAACAAGAGGAAGAGTTTAC

References

• 11318637,22960854